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Item #:
97963854
Tris-Acetate-EDTA 50X (TAE Buffer 50X), 500mL, One Bottle
Item #:
97963854
AUD 128
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What Stands Out
High Concentration
This TAE Buffer 50X offers superior concentration, making it ideal for precise electrophoresis applications, ensuring optimal performance in DNA and RNA separation experiments.
Convenient Packaging
Packaged in a sturdy 500mL bottle, it provides ample supply for multiple experiments, minimizing the need for frequent repurchases and ensuring user convenience.
Versatile Use
Suitable for various molecular biology applications, this buffer streamlines workflows, accommodating a wide range of protocols in laboratories focused on DNA and RNA research.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 1.5 pounds (680 grams) |
| Volume | 500 ml |
| Brand Name | SAPRA Biomedicals |
| Product Grade | Reagent |
| Manufacturer | SAPRA Biomedicals |
| Included Components | Tris Base - 121g, EDTA - 18.6g, Glacial Acetic Acid - 28.55ml, DI Water |
| Part Number | TAE-50-16688459 |
| Brand | SAPRA Biomedicals |
| Item Volume | 500 ml |
| Package Information | Bottle |
Who Should Buy?
-
Molecular Biologists
Ideal for DNA electrophoresis, this buffer supports high-quality results in gel separation for molecular cloning.
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Research Laboratories
Suitable for various laboratory applications, enhancing experimental accuracy in genetic studies and research initiatives.
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Educational Institutions
Perfect for teaching purposes, helping students understand electrophoresis techniques in practical lab environments.
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Non-Scientific Users
Not suitable for casual or non-scientific applications, as it requires specific knowledge for effective use.
Product Description
Tris-Acetate-EDTA 50X (TAE Buffer 50X), 500mL, One Bottle
Have any Query? Chat with us
Customer Questions & Answers
-
Question:
What is Tris-Acetate-EDTA (TAE) Buffer used for?
Answer: Tris-Acetate-EDTA (TAE) Buffer is primarily used in molecular biology, particularly for the electrophoresis of nucleic acids such as DNA and RNA. The buffer helps maintain a stable pH environment during the separation process, ensuring that the nucleic acids retain their integrity. Scenarios for use include preparing agarose gels for gel electrophoresis or running samples through a gel to check for purity and size. -
Question:
Is the 50X concentration of TAE Buffer suitable for immediate use?
Answer: No, the 50X concentration of TAE Buffer is not suitable for immediate use and must be diluted before application. To prepare a 1X working solution, you need to dilute the 50X buffer with distilled water in a 1:49 ratio. This concentration is optimal for running gels, ensuring efficient resolution of nucleic acid samples during electrophoresis. -
Question:
What volume of TAE Buffer is sufficient for a standard gel electrophoresis?
Answer: For a standard gel electrophoresis, a common volume used is approximately 50-100 mL of 1X TAE Buffer, depending on the dimensions of the gel tray. This volume ensures proper coverage of the gel and allows for effective migration of nucleic acids. It's essential to have enough buffer to maintain the ideal ionic strength and pH during the process. -
Question:
How should TAE Buffer be stored after opening?
Answer: After opening, TAE Buffer should be stored at room temperature or in a cool dry place, though refrigeration can help prolong its shelf-life. Make sure to keep the bottle tightly sealed and protected from light to prevent degradation. When properly stored, the buffer remains effective for several months, ensuring reliable results for your experiments. -
Question:
What are the advantages of using TAE Buffer over other buffers like TBE?
Answer: One of the primary advantages of using TAE Buffer over TBE Buffer is that TAE provides better resolution for larger DNA fragments. Additionally, TAE is less toxic than TBE, which often contains boric acid. These factors make TAE Buffer a preferred choice, especially for applications in molecular cloning or where high-quality results are required, such as DNA fragment analysis. -
Question:
Can I use TAE Buffer for both DNA and RNA techniques?
Answer: Yes, TAE Buffer can be utilized for both DNA and RNA applications. It is particularly effective for the electrophoresis of RNA, as it provides a conducive environment to avoid RNA degradation. Researchers often use TAE when analyzing RNA samples to ensure a stable pH and ionic strength, leading to reliable separation of nucleic acids. -
Question:
What materials do I need to prepare for using TAE Buffer in gel electrophoresis?
Answer: To prepare for using TAE Buffer in gel electrophoresis, you will need agarose gel, a gel casting tray, combs for sample wells, electrophoresis apparatus, and a power supply. You also require a micropipette and colorful DNA loading dye for adding your nucleic acid samples. These materials enable you to create the gel, load samples, and run electrophoresis seamlessly. -
Question:
Is TAE Buffer compatible with different agarose concentrations?
Answer: Yes, TAE Buffer is compatible with various agarose concentrations, typically ranging from 0.5% to 2%. The choice of agarose concentration depends on the size of the nucleic acids you wish to separate. Lower concentrations are better for larger fragments, while higher concentrations are suited for smaller DNA molecules or precise resolution requirements. -
Question:
What color indicates TAE Buffer's effectiveness?
Answer: While TAE Buffer itself is typically colorless, you can enhance its functionality by adding a loading dye to the samples before application. The loading dye usually appears blue, helping visualize sample loading and migration during electrophoresis. This visual cue assists you in monitoring the electrophoresis process, ensuring that the nucleic acids are adequately migrating through the gel. -
Question:
Where can I buy Tris-Acetate-EDTA 50X (TAE Buffer 50X), 500mL, One Bottle in Nauru?
Answer: You can buy Tris-Acetate-EDTA 50X (TAE Buffer 50X), 500mL, One Bottle from Ubuy in Nauru. Ubuy offers a reliable platform to purchase laboratory supplies and reagents, including TAE Buffer, ensuring you have access to quality products for your research needs.
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Features & Benefits
- Prepare a 1X solution easily with a simple mixing ratio.
- Maintains a constant pH for reliable experimental conditions.
- Ideal for immunodetection assays like Western Immunoblotting.
- Store between 2 to 30 ºC for optimal longevity.
- Has a shelf life of 24 months from the date of purchase.
- Designed for research use only, not for human or clinical applications.
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